Abstract
Vitamin B12 assays that depend on the competition of endogenous cobalamin (Cbl) in a sample with labeled Cbl for binding to intrinsic factor (IF) must effectively denature or inactivate any Cbl-binding proteins and autoantibodies to IF that are present in the sample. Blocking antibodies specific for IF are present in more than one-half of all patients with pernicious anemia. The alkaline hydrolysis denaturation step, coupled with 37 °C incubation, has been shown to inactivate …
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