Abstract
Background: Diabetics have many disabilities, including neuronal damege hippocampus, and BDNF is an effective factor in this field. This factor has the most impact on the hippocampus, also TNF-α is an inflammatory factor that increases in diabetes, the purpose of this study was to investigate the effect of 6 weeks of High Intensity interval training on the gene expression of brain-derived neurotrophic factor (BDNF) in the hippocampus of male Wistar rats. Methods: For this study, 48 male Wistar rats (4 weeks old) with 150± 10 g weight were categorized in 4 groups of: diabetic rats (n= 12), exercise diabetic (n= 12), control (n= 12) and exercise control (n= 12). For induction of diabetes, peritoneal injection of STZ solution (50 mg/kg) was used. After a week of familiarization with the environment and practice, high Intensity interval training protocol consisted of 3 days of training per week for 6 weeks with 50% to 110% of maximum oxygen consumption was performed. 24 hours after the completion of the exercise, the functional test was taken and the animals were autopsy 48 hours after the functional test. Finally, BDNF gene expression was evaluated using Real Time PCR technique. Results: Regarding the results, it was found that the high Intensity interval training intensified the expression of the BDNF gene and decreased the expression of the TNF-α protein. also The weight gain of the hippocampus has been observed along with increased expression of BDNF gene in the diabetic high Intensity interval training group. Conclusion: Exercise can help prevent hippocampus tissue loss, and also prevent memory damage that caused by diabetes. Also, high Intensity interval training are effective in maintaining the physical fitness of diabetics, therefore high Intensity interval training are recommended for improvement in the physical condition and life of diabetics.
Highlights
Diabetics have many disabilities, including neuronal damege hippocampus, and brain-derived neurotrophic factor (BDNF) is an effective factor in this field
Also, high Intensity interval training are effective in maintaining the physical fitness of diabetics, therefore high Intensity interval training are recommended for improvement in the physical condition and life of diabetics
تناوبی شدید بر بیان ژن عامل نوروتروفیک مشتق از مغز ( )brain-derived neurotrophic factor (BDNF)در هیپوکامپ و میزان سرمی TNF-αموشهای صحرایی نر ویستار بود. رو شکار :برای این مطالعه ،تعداد 84سر موش صحرایی نر ویستار ( 8هفتهای) با وزن 051±01گرم در 8گروه موشهای دیابتی ) ،(n=12دیابت تمرین ) ،(n=12کنترل ) (n=12و کنترل تمرین ( )n=12دسته بندی شدند .جهت القا دیابت از روش تزریق صفاقی محلول 05( STZمیلیگرم بر کیلوگرم) استفاده گردید .بعد از یک هفته آشناسازی با محیط و تمرین ،پروتکل تمرین تناوبی شدید شامل 3روز تمرین در هفته به مدت 6هفته با شدت 51تا 001درصد حداکثر اکسیژن مصرفی انجام شد 48 .ساعت بعد از اتمام تمرین ،آزمون عملکردی گرفته شده و 84ساعت بعد از آزمون عملکردی ،حیوانات تشریح شدند.
Summary
Diabetics have many disabilities, including neuronal damege hippocampus, and BDNF is an effective factor in this field.
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