Abstract
Hepatoblastoma (HB) is a kind of tumor that occurs frequently in children and is highly malignant. Here, the function of ROS modulator 1 (ROMO1) was identified in the development of HB. In this study, the mRNA expression of ROMO1 was measured by RT-qPCR. Colony formation assay, MTT assay, and flow cytometric analysis were applied to detect cell viability. The cell migrative and invasive ability was measured by wound healing and transwell assays. Tumor xenografts were performed to examine tumor growth. The results showed that upregulation of ROMO1 was identified in liver hepatocellular carcinoma (LIHC) tissues and predicted poor prognosis in LIHC patients. And ROMO1 expression was also increased in HB tissues and cells. Functionally, ROMO1 knockdown restrained cell viability, migration, and invasion in HB. In addition, knockdown of ROMO1 was found to suppress tumor formation in vivo. In conclusion, upregulation of ROMO1 promotes tumor growth and cell aggressiveness in HB.
Highlights
Hepatoblastoma (HB) develops from hepatocyte precursor cells and is a malignant embryonic tumor with multiple differentiation modes [1]. e cause of HB is not clear
We found that ROS modulator 1 (ROMO1) expression was increased in most cancers (P < 0.05, Figure 1(a))
liver hepatocellular carcinoma (LIHC) patients with higher ROMO1 expression were found to have lower overall survival (OS) and disease-free survival (DFS) than patients with low ROMO1 expression (P < 0.05, Figures 1(c) and 1(d)). ese results demonstrate that ROMO1 is upregulated in LIHC tissues
Summary
Hepatoblastoma (HB) develops from hepatocyte precursor cells and is a malignant embryonic tumor with multiple differentiation modes [1]. e cause of HB is not clear. Hepatoblastoma (HB) develops from hepatocyte precursor cells and is a malignant embryonic tumor with multiple differentiation modes [1]. HB is the most common liver malignant tumor in children, accounting for 90% of the primary malignant tumors of the liver in children. The early symptoms of HB are not obvious, and the child usually looks good. The tumor grows rapidly, and an abdominal mass may appear, which is the most common symptom of HB [5]. E treatment of HB is mainly surgery combined with chemotherapy. If the patient is actively treated, the overall survival rate of HB is about 80%, and the prognosis is good [6]. Erefore, early detection, early diagnosis, and early treatment are extremely important for improving the survival rate of patients with HB If the patient is actively treated, the overall survival rate of HB is about 80%, and the prognosis is good [6]. erefore, early detection, early diagnosis, and early treatment are extremely important for improving the survival rate of patients with HB
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