High efficiency direct shoot organogenesis from leaf segments of Aerva lanata (L.) Juss. ex Schult by using thidiazuron.

K Varutharaju,C Thilip,A Aslam,C Soundar Raju,A Shajahan

doi:10.1155/2014/652919

Abstract

An efficient protocol for direct shoot organogenesis has been developed for the medicinal plant Aerva lanata (L.) Juss. ex Schult. Regeneration was achieved from leaf segments of 20 days old in vitro plantlets raised on Murashige and Skoog (MS) medium containing 0.25–2.0 mg L−1 thiadiazuron (TDZ), 3% sucrose, and 0.8% agar. After 21 days of culture incubation, maximum number of shoot organogenesis (23.6 ± 0.16) was obtained on medium containing 1.0 mg L−1 TDZ. The shoots were able to produce in vitro flowers on medium containing 1.0 mg L−1 TDZ in combination with 0.25–0.5 mg L−1 α-naphthaleneacetic acid (NAA). Histological observation showed that the epidermal cells of the leaf explants exhibited continuous cell division led to formation of numerous dome shaped meristematic protrusions and subsequently developed into adventitious shoots. Upon transfer of shootlets to half strength MS medium containing 1.0 mg L−1 indole-3-butyric acid (IBA), around 86% of the regenerated shoots formed roots and plantlets. Rooted plants were hardened and successfully established in the soil at the survival rate of 92%. The regeneration protocol developed in this study provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large scale production of its medicinally active compounds and genetic transformations for further improvement.

Highlights

Aerva lanata (L.) Juss. ex Schult., a medicinal herb belonging to the family Amaranthaceae, is commonly called Polpala
Direct shoot organogenesis from leaf segments represent a promising tool for mass propagation as well as genetic transformation system
Since there is no previous information on shoot development from leaf segments of A. lanata, there has been a new report

Summary

Introduction

Aerva lanata (L.) Juss. ex Schult., a medicinal herb belonging to the family Amaranthaceae, is commonly called Polpala. The bioactive active compounds responsible for the above pharmacological activities are β-carboline, β-sitosterol, palmitic acid, alpha amyrin, aervin, methyl aervine, and aervoside [5, 6]. The requirement of this medicinal herb is presently met from the natural populations. There are few reports on in vitro regeneration of A. lanata, which are restricted to adventitious plantlet formation from shoot tip and nodal segments [7]. Direct shoot organogenesis from leaf segments represent a promising tool for mass propagation as well as genetic transformation system. In the present study, an attempt has been made to develop an efficient direct regeneration system using leaf segments for A. lanata

Methods

Results

Conclusion