High dietary selenium intake alters tissue lipid and protein profiles and metabolisms of growing pigs

Abstract

We previously demonstrated that high dietary Se intake induced mild hyperinsulinemia and up‐regulated lipogenesis and protein synthesis signaling. The present study was conducted to reveal impacts of the high Se intake on tissue lipid and protein profiles and metabolic regulations. A total of 12 weanling pigs were divided into 2 groups (n = 6/group), and fed a corn‐soybean meal basal diet supplemented with Se (as Se enriched yeast) at normal (0.3 mg/kg) or high (3.0 mg/kg) concentration for 11 wk. While the high Se diet elevated (P < 0.05) total triglycerides and cholesterol concentrations in both liver and adipose tissues and non‐esterified fatty acid concentrations in the liver, it showed little effect on any of these measures in the muscle. The high Se diet enhanced (P < 0.05) myristic acid, margaric acid, and eicosapentaenoic acid concentrations, but decreased (P < 0.05) arachidonic acid concentrations in the liver. Concentrations of total protein in the liver and muscle as well as of 3 amino acids (Asp GLu, and Ala) were elevated (P < 0.05) by the high Se diet. Meanwhile, hepatic phosphoenolpyruvate carboxykinaseactivity and total and phosphorylated AMP‐activated protein kinase were up‐regulated (P < 0.05) by the high Se diet, although such effects were less in the muscle. In conclusion, high dietary Se intake seemed to exert differential impacts on the overall energy metabolism and biosynthesis of lipids and proteins in the liver, muscle, and adipose tissue of growing pigs. (NIH DK 53018).