Abstract
Flesh color (FC), skin color (SC), and anthocyanin content (AC) are three important traits being used for commodity evaluation in purple-fleshed sweet potato. However, to date, only a few reports are available on the inheritance of these traits. In this study, we used a biparental mapping population of 274 F1 progeny generated from a cross between a dark purple-fleshed (Xuzishu8) and white-fleshed (Meiguohong) sweet potato variety for genetic analyses. Correlation analysis showed a significant positive correlation among AC, SC, and FC. Medium-to-high heritability was observed for these traits. We detected single nucleotide polymorphisms (SNPs) by specific length amplified fragment sequencing (SLAF-seq) with the average sequencing depth of 51.72 and 25.76 for parents and progeny, respectively. Then we constructed an integrated genetic map consisting of 15 linkage groups (LGS) of sweet potato spanning on 2,233.66 cm with an average map distance of 0.71 cm between adjacent markers. Based on the linkage map, ten major quantitative trait loci (QTLs) associated to FC, SC, and AC were identified on LG12 between 0 and 64.97 cm distance, such as one QTL for SC and FC, respectively, which explained 36.3 and 45.9% of phenotypic variation; eight QTLs for AC, which explained 10.5–28.5% of the variation. These major QTLs were highly consistent and co-localized on LG12. Positive correlation, high heritability, and co-localization of QTLs on the same LG group confirm the significance of this study to establish a marker-assisted breeding program for sweet potato improvement.
Highlights
IntroductionIn the past three decades, various types of molecular markers have been developed and utilized by researchers for crop improvement purposes, such as random amplified polymorphic DNA (RAPD) (Ukoskit et al, 1997), amplified fragment length polymorphism (AFLP) (Kriegner et al, 2003; Cervantes-Flores et al, 2008; Zhao et al, 2013; Ma et al, 2020), simple sequence repeats (SSR) (Zhao et al, 2013; Kim et al, 2017; Ma et al, 2020), and single-nucleotide polymorphism (SNP) (Zhang Z. et al, 2015; Shirasawa et al, 2017; Mollinari et al, 2020)
Sweet potato [Ipomoea batatas (L.) Lam., I. batatas (2n = 6x = 90)] is an important root crop worldwide owing to its rich nutritional contents, high yield, and wide range of adaptability (Ma et al, 2020)
The results showed that the anthocyanin content (AC) content was predominantly conditioned by the genotype effect (Figure 1F)
Summary
In the past three decades, various types of molecular markers have been developed and utilized by researchers for crop improvement purposes, such as random amplified polymorphic DNA (RAPD) (Ukoskit et al, 1997), amplified fragment length polymorphism (AFLP) (Kriegner et al, 2003; Cervantes-Flores et al, 2008; Zhao et al, 2013; Ma et al, 2020), simple sequence repeats (SSR) (Zhao et al, 2013; Kim et al, 2017; Ma et al, 2020), and single-nucleotide polymorphism (SNP) (Zhang Z. et al, 2015; Shirasawa et al, 2017; Mollinari et al, 2020). The utilization of traditional markers, such as AFLPs and RAPDs, is restricted due to their limited quantity, repeatability, and stability (Yagi, 2018)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
