Abstract
Microencapsulation of lipase from Yarrowia lipolytica IMUFRJ 50682 was performed by ionotropic gelation with sodium alginate. Sodium alginate, calcium chloride and chitosan concentrations as well as complexation time were evaluated through experimental designs to increase immobilization yield (IY) and immobilized lipase activity (ImLipA) using p-nitrophenyl laurate as substrate. To adjust both parameters (IY and ImLipA), the desirability function showed that microcapsule formation with 3.1%(w/v) sodium alginate, 0.19%(w/v) chitosan, 0.14 M calcium chloride, and 1 min complexation time are ideal for maximal immobilization yield and immobilized lipase activity. A nearly twofold enhancement in Immobilization yield and an increase up to 280 U/g of the lipase activity of the microcapsules were achieved using the experimental design optimization tool. Chitosan was vital for the high activity of this new biocatalyst, which could be reused a second time with about 50% of initial activity and for four more times with about 20% of activity.
Highlights
IntroductionEnzymatic immobilization arose primarily as a response to the need of reusing expensive enzymes in industrial processes [1]
Immobilization is a powerful tool to improve enzyme properties
The results show that sometimes high immobilization yield (IY) is not accompanied by a high
Summary
Enzymatic immobilization arose primarily as a response to the need of reusing expensive enzymes in industrial processes [1]. Currently this technology solves the problem associated with enzyme recovery, and, if used properly, improves many other characteristics of enzymes, such as stability, selectivity, activity, resistance to inhibitors and purity, among others [2,3]. Y. lipolytica is known to possess 16 paralogs of genes coding for lipase, out of which Lip is the main extracellular lipase secreted by this yeast [8]. The structural feature of Lip is characterized by the presence of a mobile subdomain, called lid, whose conformational changes control the access of substrate molecules to the catalytic center [9]
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