Abstract
Herein, we developed a sensitive, selective, efficient, and low-cost method for higenamine (HG) detection. This method employs high-performance liquid chromatography with a chemiluminescence detector (HPLC-CL) coupled with an AgIII-luminol system. Lotus plumule and human urine samples were purified using a mixed weak cation exchange column (PWCX) solid-phase extraction cartridge, and a C18 column was employed for chromatographic separation using an aqueous solution of acetonitrile and 0.1% formic acid as the mobile phase. Under optimal conditions, the chemiluminescence (CL) signal decreased linearly with the logarithm of the concentration of HG in the range of 5-800 ng mL-1 . The limit of detection (LOD) and limit of quantification (LOQ) of HG in the urine sample were 0.41 and 1.35 ng mL-1, respectively. The average recovery of HG in the urine and lotus plumule samples at three levels ranged from 80.02 to 110.40% with a relative standard deviation of less than 10%. Furthermore, we proposed a mechanism for CL signal reduction in the system. Finally, the urinary pharmacokinetics of HG in humans were studied to provide valuable information and reference for athletes on medication or diet during events.
Published Version
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