Abstract
Objective By effectively interfering with hypoxia inducible factor-1 (HIF-1) in colon cancer SW480 cells to observe the effect of HIF-1 silencing on apoptosis of tumor cells under hypoxia. Methods A three-gas incubator was used to induce the hypoxia environment. SW480 cells were transfected with pGenesil-11 plasmid to silence HIF-1. Under the transmission electron microscopy the morphological changes of tumor cells were observed. Cell apoptosis was detected by fluorescent dye Hcechst 33258 and DNA Ladder. Results As compared with the interference efficacy at the 3rd day (0.19±0.02) and 15th (0.20±0.03) ,the best interference efficacy was achieved at the 7th day (0.16±0.02,P <0.05). In colon cancer SW480 cells of the positive interference group, the formation of apoptotic bodies and other morpho-logical changes of apoptosis were observed under the electron microscopy; Apoptosis fluorescence detection showed strong blue fluorescence staining, and nuclear fragmentation was seen; DNA Ladder detection showed a typical apoptosis ladder. Conclusion HIF-1 plays the role against hypoxia-induced apoptosis in tumor cells. Key words: Colon carcinoma; RNA interference; Apoptosis
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
