Abstract

Objective To investigate the impacts of MYCN expression alternation on apoptosis and proliferation in MYCN-amplifieation of neuroblastoma cell line.Methods Neuroblastoma cell line LAN-1 was euhured and transferred by MYCN-siRNA.The gene silencing was tested by Real time PCR and western-blot.The apoptosis and expression of neuron-specific enolase of tumor cells were measured by EIASA and western-blot,respectively.Results After transferring of siRNA for 12 h and 24 h,the densimeter recorder of MYCN mRNA was 0.53± 0.10 (vs.control 1,P＜0.05),0.28±0,09 (vs.control 1.12± 0.31,P＜0.05),respectively.Western-blot showed that the densitometric quantification of MYCN protein was 0.76±0.13 (vs.control 1.25± 0.21,P＜0.05) 12 h after siRNA transferring,while that of 24h was 0.44 ±0.07 (vs.control 1.39± 0.29,P＜0.05).After the suppression of MYCN expression,the apoptosis of tumor cells was enhanced (1.90 ±0.12 vs 1.13±0.09,P＜0.05),and the expression of neuron-speeifie enolase was increased (1.04 ± 0.14 vs 0.47 ±0.10,P＜0.05).Conclusions RNA interference could inhibit MYCN expression in vitro and promote the apoptosis and differentiation of tumor cells. Key words: Neuroblastoma; Apoptosis

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