Abstract
1. 1. d-Glucose (0.5–16.7 mM) preferentially stimulates aerobic glycolysis and d-[3,4- 14C] glucose oxidation, relative to d-[5- 3H]glucose utilization in rat pancreatic islets, the concentration dependency of such a preferential effect displaying a sigmoidal pattern. 2. 2. Inorganic and organic calcium antagonists, as well as Ca 2+ deprivation, only cause a minor decrease in the ratio between d-[3,4- 14C]glucose oxidation and d-[5- 3H]glucose utilization in islets exposed to a high concentration of the hexose (16.7 mM). 3. 3. Non-glucidic nutrient secretagogues such as 2-aminobicyclo[2,2,1]heptane-2-carboxylate (BCH), 2-ketoisocaproate and 3-phenylpyruvate fail to stimulate aerobic glycolysis and d[3,4- 14C]glucose oxidation in islets exposed to 6.0 mM d-glucose. Nevertheless, BCH augments [1- 14C]pyruvate and [2- 14C]pyruvate oxidation. 4. 4. The glucose-induced increment in the paired ratio between d-[3,4- 14C]glucose oxidation and d-[5- 3H]glucose utilization is impaired in the presence of either cycloheximide or ouabain. 5. 5. These findings suggest that the preferential effect of d-glucose upon aerobic glycolysis and pyruvate decarboxylation is not attributable solely to a Ca 2+-induced activation of FAD-linked glycerophosphate dehydrogenase and/or pyruvate dehydrogenase, but may also involve an ATP-modulated regulatory process.
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