Abstract
Myrmecia incisa is a valuable microalga worth investigating as it is rich in arachidonic acid (ArA), a polyunsaturated fatty acid that is essential for human health. The present study developed a two-stage cultural strategy for M. incisa. In stage Ⅰ, heterotrophic mode was conducted for the purpose of high algal biomass production. By determining the optimal cultural parameters including dissolved oxygen level, inoculum size, glucose concentration and pH values, the biomass of M. incisa was up to 10.86 g L−1, representing the highest report on the heterotrophic growth of M. incisa so far. In stage Ⅱ, the algae were transferred to phototrophic mode and subjected to nutrient deficiency for the purpose of rapid ArA accumulation. As a result, the final ArA content was 11.95% of dry cell weight with the maximum productivity being 48.52 mg L−1d−1. In addition, it was found that 85.12% of ArA was stored in neutral lipids, which is beneficial for the downstream separation and extraction. Findings of the present study strongly demonstrated the superiority of M. incisa as a promising feedstock for ArA-rich oil.
Published Version
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