Abstract
This chapter describes the protocol for heterologous expression of Phytophthora proteins in the yeast Pichia pastoris. Two methods to prepare the constructs for expression are described, using two different strains of P. pastoris, as well as methods for protein expression and purification by immobilized metal ion affinity (IMAC). The combined protocol was used to successfully produce a Phytophthora pluvialis small, secreted cell death elicitor protein and a Phytophthora agathidicida glycoside hydrolase 12, PaXEG1, also a cell death-inducing protein.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call