Heterogeneity of toad bladder granular cell luminal membranes Distribution of filipin-sterol complexes in freeze-fracture

Abstract

Glutaraldehyde-fixed toad urinary bladders were incubated with the sterol specific antibiotic, filipin, and the distribution of the resulting filipin-sterol complexes was studied in freeze-fracture replicas. While the luminal membrane of some cells had a heavy, homogenous labelling, most had clusters of filipin-sterol complexes scattered on an otherwise poorly labelled membrane. These clusters were seen to overlie cytoplasmic granules, which are found just beneath the luminal plasma membrane of this cell type, and which were always heavily labelled. These results suggest that (a) the granule membranes have a high cholesterol content and (b) sites of interaction between granule and luminal membranes might be enriched in cholesterol. In addition, filipin-sterol complexes were absent from vasopressin-induced particle aggregates on the granular cell luminal membrane.