Heteroagglomeration of oxide nanoparticles with algal cells: effects of particle type, ionic strength and pH.

Abstract

Discharged oxide nanoparticles (NPs) have been shown toxic to unicellular algae, yet the research on heteroagglomeration between NPs and cells as an important precondition of the toxicity is scarce. This study for the first time investigated heteroagglomerations between various NPs and algal cells (Chlorella pyrenoidosa) and analyzed influencing factors including pH and ionic strength (IS) through cosettling experiment, transmission electron microscopic (TEM) observation, and Derjaguin–Landau–Verwey–Overbeek (DLVO) calculation. The examined NPs included anatase and rutile TiO2, microporous and spherical SiO2, and α-form and β-form Al2O3. The results of cosettling experiments coincided well with the TEM observations, whereas the DLVO theory could only partly explain the NP–cell heteroagglomerations. The NP–cell heteroagglomeration for rutile TiO2 and β-form Al2O3 was weak and insensitive to pH or IS. Preferential heteroagglomeration occurred at low pH or high IS for microporous SiO2, while marked heteroagglomeration only occurred under the neutral and low IS condition for anatase TiO2. The heteroagglomeration for spherical SiO2 was insensitive to pH but increased with increasing IS, while the heteroagglomeration for α-form Al2O3 occurred at low pH and irrelevant to IS. The work will shed new light on the bionano interfacial interaction and help to understand biological effects of NPs.