Hesperidin Alleviates Cecal Ligation and Puncture-Induced Lung and Kidney Injuries

Abstract

Objective: The potential useful features of hesperidin (Hes) was examined against lung and kidney injuries triggered by cecal ligation and puncture (CLP) in rats in current study. Materials and Methods: 32 Wistar Albino male rats were randomly allocated as sham, CLP, Hes 100 mg/kg and Hes 200 mg/kg groups. For the CLP process under anesthesia, the abdominal area was shaved and cleaned. The cecum was tied with a 4.0 suture and pierced by 18-gauge needle. Using this method, the experimental sepsis model was created. The lung and kidney tissues were removed at the end of the experiment. Biochemical and immunohistochemical analyzes were performed. Hes was administered by oral gavage at the doses of 100 and 200 mg/kg for 15 days. Results: Total oxidant status (TOS), malondialdehyde (MDA) and oxidative stress index (OSI) levels, myeloperoxidase (MPO) activity raised significantly while superoxide dismutase (SOD) and total antioxidant status (TAS) values declined in CLP group compared to sham group in lung and kidney tissues. On the contrary, SOD and TAS levels increased while MPO activity, TOS, OSI and MDA levels decreased due to Hes treatments. Caspase-3 and LC3B immunopositivity raised significantly in CLP group compared to sham group in lung and kidney tissues while decreased was observed in Hes treatment groups. Conclusion: In this study, in the light of our biochemical and immunohistochemical results that it has been detected that, Hes demonstrated an effective protection against CLP-induced lung and kidney tissue injuries in rats. Key Words: Sepsis, Hesperidin, Lung, Kidney, Oxidative stress, Autophagy