Abstract
Hepatitis developed in newborn Swiss mice following intraperitoneal inoculation with herpes simplex virus. The titer of virus in the liver rapidly rose and reached a maximum level (10 6·3 TCD 50/gm of liver) by day 2; virus was not yet detectable in the blood at this time. Our findings suggested that virus directly penetrated the liver capsule and invaded and destroyed adjacent parenchymal cells. Light-microscopic examination revealed a layer of necrotic hepatic cells which were contiguous with the liver capsule and also extended along connective tissue septa of the liver. Electron-microscopic examination revealed that serosal cells of the liver capsule, and the adjacent hepatocytes, were infected. Extracellular virions intermixed with necrotic cell debris were seen along the liver capsule; virus particles were seen also within the basement membrane separating the capsule from underlying parenchyma. Nonenveloped virions (100 mμ in diameter) developed within nuclei of hepatocytes and frequently appeared to differentiate in or nearby foci of polygonal-shaped ring forms (35–45 mμ in diameter) which resembled virus cores. Plexiform tubular structures (about 10 mμ in diameter) were occasionally seen in foci of developing virions, but their significance is unclear.
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