Hepatoprotective effect of eugenol on laboratory rats under simulated toxicosis

Abstract

Recently, in the literature, there is often a mention of bioflavonoid hepatoprotectors. These include: silibinin, silidianin, silicristin, isosilibinin, etc.In foreign sources, there is a mention of eugenol as a remedy that can be used as a choleretic and antitoxic agent. At present, in Russia, eugenol is used as an active antiseptic, and also has an analgesic effect.Eugenol is a pale yellow liquid with an oily consistency and a spicy aroma, slightly acidic, slightly soluble in water and highly soluble in organic solvents such as alcohol, oils, ether and chloroform.Rats (male, weight 200-250g) were injected with various doses of eugenol before the introduction of toxic doses of ethyl alcohol, according to the method. The drug was administered intragastrically. After 24 hours of fasting, the rats were randomly divided into 5 groups (n = 6). The dose of eugenol was chosen based on our preliminary screening, and taking into account the rationale for the dose in the literature. All animals were examined in an hour, conducting a biochemical analysis of the main indicators reflecting the state of the liver (bilirubin, ALT, AST) by puncture of the tail vein. All values were measured as mean ± SD, data with p<0.05 were considered significant.Studies have established that ethyl alcohol is a significant factor in gastric mucosal damage and upper gastrointestinal hemorrhage and general toxic condition. As a result of the study, it was also revealed that biochemical changes were minimal and tended to complete recovery, which indicates the presence of a hepatoprotective effect in the study drug.