HEPATOPROTECTIVE AND HISTOLOGICAL PANCREAS EFFECTS OF Sarang Banua (Clerodenrum fragrans Vent Willd) LEAF EXTRACT IN ALLOXAN-INDUCED DIABETIC RATS

Abstract

Research on anti-diabetic herbal plants and their safety continues to grow by the increasing number of diabetics, especially in Indonesia, which is rich in plant biodiversity. This study investigated the effect of Sarang banua (Clerodendrum fragrans Vent Willd) leaf extract on the hepatoprotective effect, histology of the pancreas, and serum glucose levels of white male rats (Rattus novergicus) induced diabetes by alloxan. Diabetic rats were given ethanol extract of C. fragrans leaf (100, 200, and 300 mg/kg bw), ethyl acetate extract (200, 300 mg/kg bw), and metformin (125 mg/kg) orally for 14 days. Enzym activities and glucose levels in serum were determined at the end of treatments. The histology of rat pancreas and liver were observed under an electron microscope with 10x magnification. The data obtained were analyzed by ANOVA followed by the Least Significant Difference test. The results showed that the administration of ethanolic extract of C. fragrans leaves at 100mg/kg bw significantly decreased the serum glutamicoxaloacetic transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT), and serum glucose levels in the alloxan-induced diabetic rat (p < 0.05). C. fragrans leaf extract decreased the level of histological damage liver and pancreas of alloxan-induced diabetic rats. The C. fragrans improved the protective effects on the liver, and pancreas, and decreased serum glucose in alloxan-induced diabetic rats.