Abstract
BackgroundMedicinal and aromatic plants have the potential to treat various disorders both human and animals. This present work investigated the hepatoprotective and antioxidant potential of aqueous extract of Ipomoea staphylina leaves on Wistar rats against CCl4-induced liver injury.MethodsIn vitro free radical scavenging activity of aqueous extract of Ipomoea staphylina (AIS) was estimated using ABTS [2, 2-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)} and DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radicals. Pharmacologically important phytochemicals such as phenolic compounds and flavonoids were analyzed using HPLC technique. Hepatotoxicity was induced by oral administration of CCl4 at 1 mL/kg body weight of Wistar rats twice a week for 8 weeks. Experimental rats received AIS orally at the doses of 250 and 500 mg/kg daily for 8 weeks. Silymarin, a routine hepatoprotective drug, was used as a reference drug.ResultsTotal phenolics and flavonoid content of AIS are 20.78 ± 0.86 mg of GAE/g and 17.78 ± 0.45 mg of RE/g of extract respectively. ABTS and DPPH free radical scavenging activity of AIS exhibits the IC50 value of 32.08 ± 0. 12 and 45.24 ± 0.65 μg/mL respectively. HPLC analysis of AIS showed the presence of polyphenolic compounds such as protocatechuic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, naringinin, qurecetin, rutin and flavon. Treatment with AIS restored the biochemical parameters towards normal level, prevented the occurrence of LPO and significantly (P ≤ 0.05) increased the levels of enzymatic (SOD and catalase) and non-enzymatic (reduced glutathione) antioxidants in CCl4 administered Wistar rats. Histopathology results confirmed the hepatoprotective effect of AIS.ConclusionBased on the results obtained, we suggest that the I. staphylina leaves extract have potent antioxidant and hepatoprotective activity.
Highlights
Liver provides protection to an organism against foreign particles by eliminating them, through the process of excretion [1]
The results shows that the reducing at100 μg/mL concentration had an optical density of 0.4274, which was comparatively similar to that of the standard Butylated hydroxyltoluene (BHT) with an optical density of 0.4766
Our study demonstrated that the aqueous extract of Ipomoea staphylina (AIS) leaves showed favorable potency to protect the liver damage induced by Carbon tetrachloride (CCl4), which suggested that I. staphylina can be used as a potential hepatoprotective drug
Summary
Liver provides protection to an organism against foreign particles by eliminating them, through the process of excretion [1]. In Asian traditional medicine system, I. staphylina is an extensive climber, commonly used for the treatment of liver diseases, purgation, stomach disorders, pain, inflammation, and in rheumatism [8]. The antioxidant and hepatoprotective activity of of I. staphylina has not been evaluated scientifically till now. Identification of phytocompounds from I. staphylina aquous leaves extract (AIS) and evaluate its antioxidant and hepatoprotective activity against CCl4 induced oxidative damage in Wistar rats was the main aim of this research. Medicinal and aromatic plants have the potential to treat various disorders both human and animals. This present work investigated the hepatoprotective and antioxidant potential of aqueous extract of Ipomoea staphylina leaves on Wistar rats against CCl4-induced liver injury
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