Abstract
RSM was used to determine the optimum conditions for maximum recovery of phenolics from amla seed coat powder. At optimum conditions, TPC (total phenolic content) content and % DPPH free radical scavenging activity of extract were found to be 7.02 mg GAE / mL of extract and 42.69 %, respectively. Phenolics were extracted in water, diethyl ether, butanol and ethyl acetate. % DPPH free radical scavenging (61.07 %) activity was maximum in ethyl acetate fraction. The major phenolics in ethyl acetate extract were gallic, catechin, caffeic, quercetin, p-coumaric, vanillic and chlorogenic acids. Ethyl acetate extract was further fractionated with six fractions using methanol: water by column chromatography. Fraction V showed highest DPPH scavenging activity. The phenolics in different fractions of ethyl acetate extract were also estimated by GC-MS after derivatization. Caeffic, p-coumaric, hydroxy caeffic, vanillic, gallic and p-hydroxybenzoic acid were identified. Other components like tetracontane-1,40-diol and octadecanoic acid could also be identified.
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