Abstract
Abstract Hepatitis-associated antigen (HAA) has been isolated from pooled HAA positive human serum for use as a purified HAA primary standard and in production of antiserum in guinea pigs. These reagents were used in conjunction with electrophoresis in antibody-containing agarose gel, to simultaneously screen for and measure HAA. The smallest concentration of antigen that we could detect was 2 mg/100 ml of serum; the highest concentration of antigen encountered was 170 mg/100 ml. The method was compared with the Ouchterlony double diffusion and counterelectrophoresis techniques. Several antisera against HAA are compared with each other and their titers expressed in milligrams of HAA precipitated per milliliter of antiserum.
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