Abstract
Based on available toxicological studies dealt with aquatic fauna in Egypt, none of these studies considered terrestrial stage of amphibian exposure and uptake of metal from surroundings habitat. Little is known about how contaminants affect amphibians at the population level. Three sites were selected scarily in order to comprise different types and sources of pollutants from one hand, and to prove the potential of selected animals to accumulate pollutants. The common Egyptian toad Amietophrynus Regularis was collected from different localities along the river Nile. The aim of our study was to evaluate the biochemical and histopathological biomarkers in this specie to a pollution gradient caused by domestic discharges and heavy metals and to verify the adequacy of the studied animals as appropriate bioindicator in their habitat. The results of this study revealed that deterioration of natural habitat of the selected amphibian produced manifested biochemical and histopathological changes in the liver.
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