Heparin-grafted PVA hydrogels: a material for the optical part of artificial cornea

Abstract

Heparin (Hep) was grafted to a poly(vinyl alcohol) (PVA) hydrogel surface by using the covalent grafting method. The structure of the modified hydrogel was determined from Fourier transform infrared attenuated total reflection and X-ray photoelectron spectroscopy. The thermal stability of the samples was investigated by thermogravimetry–differential thermal analysis. The effects of the concentration of (3-aminopropyl)triethoxysilane (kh550) and Hep on visible light transmittance, moisture content, equilibrium swelling, hydrophilicity and percentage of Hep sodium release of the composite hydrogel were studied. The visible light transmittance of the modified PVA hydrogel was above 94%. The time of swelling equilibrium was about 60 min and the equilibrium swelling ratio ranged from 3·0 to 3·5. The hydrophilicity was enhanced, and the static water contact angle decreased from 41 to 28°. The bioeffects of the PVA–kh550–Hep hydrogel were evaluated by studying cell adhesion and proliferation. During the adhesion assay in vitro, cell adhesion significantly decreased after the interfaces had been modified with Hep. The Cell Counting Kit-8 assay showed that the biocompatibility of the PVA–kh550–Hep hydrogel improved obviously compared to that of pure PVA. The experimental results demonstrated that the PVA–kh550–Hep hydrogel had good stability, bioactivity and biocompatibility, suggesting its potential applications in artificial corneas.