Hemolytic Activity, Cytotoxicity, and Antimicrobial Effects of Human Albumin- and Polysorbate-80-Coated Silver Nanoparticles.

Dmitry Korolev,Alexander Krutikov,Michael Galagudza,Michael Shumilo,Andrew Gorshkov,Alexander Krasichkov,Alexander Mishanin,Alexey Golovkin,Anna Domorad,Galina Shulmeyster,Anna Spiridonova

doi:10.3390/nano11061484

Abstract

In this study, we aimed to develop a technique for colloidal silver nanoparticle (AgNP) modification in order to increase their stability in aqueous suspensions. For this purpose, 40-nm spherical AgNPs were modified by the addition of either human albumin or Tween-80 (Polysorbate-80). After detailed characterization of their physicochemical properties, the hemolytic activity of the nonmodified and modified AgNPs was investigated, as well as their cytotoxicity and antimicrobial effects. Both albumin- and Tween-80-coated AgNPs demonstrated excellent stability in 0.9% sodium chloride solution (>12 months) compared to nonmodified AgNPs, characterized by their rapid precipitation. Hemolytic activity of nonmodified and albumin-coated AgNPs was found to be minimal, while Tween-80-modified AgNPs produced significant hemolysis after 1, 2, and 24 h of incubation. In addition, both native and Tween-80-covered AgNPs showed dose-dependent cytotoxic effects on human adipose-tissue-derived mesenchymal stem cells. The albumin-coated AgNPs showed minimal cytotoxicity. The antimicrobial effects of native and albumin-coated AgNPs against S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp. were statistically significant. We conclude that albumin coating of AgNPs significantly contributes to improve stability, reduce cytotoxicity, and confers potent antimicrobial action.

Highlights

IntroductionThe growing incidence of antimicrobial drug resistance in a number of bacterial species is becoming a substantial barrier for the effective treatment of many infectious diseases
After confirming minimal cytotoxicity of albumin-coated AgNPs, we studied their antimicrobial activity against some of the bacterial species belonging to the ESKAPE group—in particular, S. aureus, K. pneumonia, P. aeruginosa, Corynebacterium spp., and Acinetobacter spp.—and compare it to that of uncovered AgNPs
The plates were incubated at presented as mean ± standard deviation (SD)

Summary

Introduction

The growing incidence of antimicrobial drug resistance in a number of bacterial species is becoming a substantial barrier for the effective treatment of many infectious diseases. The infections caused by these types of pathogens are associated with higher morbidity and mortality, increased risk of adverse outcomes after medical interventions, increased treatment costs, and prolonged hospital stay [3]. These factors result in a huge economic burden on the healthcare systems. Antimicrobial resistance is recognized by the World Health Organization as one Nanomaterials 2021, 11, 1484.

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