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Abstract
The Raman spectrum of a single erythrocyte captured by a microfluidic chip was recorded to determine the conformation of hemoglobin under conditions similar to the hemodynamics of a blood vessel. Amplitude changes in the Raman spectrum at 1355, 1375, 1552, 1620, 1585 and 1637 cm−1 reflect changes in pO2 due to O2 binding to hemoglobin heme.
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