Hemocytes of zebra mussels (Dreissena polymorpha) are relevant cells for the monitoring of environmental genotoxicity by the comet assay.

Abstract

Event Abstract Back to Event Hemocytes of zebra mussels (Dreissena polymorpha) are relevant cells for the monitoring of environmental genotoxicity by the comet assay. Marc Bonnard1*, Gabrielle Magniez1, Alban Franco1, Laurence Delahaut1, Isabelle Bonnard1, Damien Rioult1, Sandrine Joachim2, Jean-Marc Porcher2 and Alain Geffard1 1 Université de Reims Champagne Ardenne , France 2 INERIS, France The measure of DNA integrity by the single cell gel electrophoresis (SCGE) or comet assay is especially recommended for its sensitivity and its capacity for detecting different types of damages. Therefore, it has been applied in environmental genotoxicity in a variety of organisms. It appears today necessary to define both reference and threshold levels of DNA damage, for their application in in situ biomonitoring. However, little is known about the influence of both biological (sex, reproduction status) or external (temperature…) confounding factors on the measure of DNA damage by the comet assay. These variables need to be taken into account if the robustness of the assay is to be established (Jha, 2008). In the zebra mussel Dreissena polymorpha (recommended as a sentinel species in the evaluation of freshwater quality) the measure of DNA damage by the comet assay is mainly performed on hemocytes, which are circulating cells involved in key physiological functions such as immunity, homeostasis, detoxication…. This communication will present and discuss results from an innovative study about the variability of the baseline level of DNA damage in hemocytes of mussels encaged for one year in the canal de l’Aisne à la Marne (Reims), according to their sex and their reproductive status. The sensitivity and the suitability of hemocytes in the evaluation of environmental genotoxicity will also be discussed, referring to observations during a 6 month-exposure of mussels in mesocosms to environmentally realistic concentrations of carbamazepine. References Jha, AN (2008). Ecotoxicological applications and significance of the comet assay. Mutagenesis, 23(3), 207-221. Keywords: Hemocytes, Zebra mussels, confounding factors, mesocosm, Carbamazepine Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Oral Presentation Topic: Ecogenotoxicology Citation: Bonnard M, Magniez G, Franco A, Delahaut L, Bonnard I, Rioult D, Joachim S, Porcher J and Geffard A (2015). Hemocytes of zebra mussels (Dreissena polymorpha) are relevant cells for the monitoring of environmental genotoxicity by the comet assay.. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00065 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 May 2015; Published Online: 23 Jun 2015. * Correspondence: Dr. Marc Bonnard, Université de Reims Champagne Ardenne, Reims, France, marc.bonnard@univ-reims.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marc Bonnard Gabrielle Magniez Alban Franco Laurence Delahaut Isabelle Bonnard Damien Rioult Sandrine Joachim Jean-Marc Porcher Alain Geffard Google Marc Bonnard Gabrielle Magniez Alban Franco Laurence Delahaut Isabelle Bonnard Damien Rioult Sandrine Joachim Jean-Marc Porcher Alain Geffard Google Scholar Marc Bonnard Gabrielle Magniez Alban Franco Laurence Delahaut Isabelle Bonnard Damien Rioult Sandrine Joachim Jean-Marc Porcher Alain Geffard PubMed Marc Bonnard Gabrielle Magniez Alban Franco Laurence Delahaut Isabelle Bonnard Damien Rioult Sandrine Joachim Jean-Marc Porcher Alain Geffard Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.