Hematopoietic effects of paeoniflorin and albiflorin on radiotherapy and chemotherapy-induced anemia mice

Abstract

Paeoniflorin (PF), a monoterpene glycoside isolated from P. lactiflora, possesses a variety of pharmacological activities. However, albiflorin (AF), another constituent regarded as a characteristic one, has not been well studied. This study aimed to investigate the hematopoietic effects of AF and PF on anemia mice induced by radiotherapy or chemotherapy and to explore the underlying mechanisms. The anemia mice were irradiated at a dose of 2.5 Gy using cobalt-60 gamma resources or intraperitoneally injected with cyclophosphamide (160.0 mg/kg). The numbers of blood cells from peripheral blood were counted. The thymus index and spleen index were also measured. In addition, of the chemotherapy-induced mice, the levels of TNF-α, GM-CSF, IL-3 in serum were measured by RIA. AF and PF significantly increased the numbers of peripheral blood cells and reversed the atrophy of thymus and spleen. Furthermore, AF and PF increased the levels of GM-CSF and IL-3 and reduce the level of TNF-αin serum.. Our results suggest that AF and PF may promote the recovery of bone marrow hemopoietic function in a myelosuppressed mouse model.