Hematology, Plasma Biochemistry, Protein Electrophoresis, and Pathogen Surveillance in Headstarted and Wild-Reared Populations of Blanding's Turtles (Emydoidea blandingii) in Three Northern Illinois, USA, Counties.

Abstract

Blanding's turtles (Emydoidea blandingii) are a species of conservation concern throughout their natural range. Headstarting is a common chelonian conservation technique in which neonates are reared in managed-care settings before release, but health assessments are rarely incorporated. From 2020 to 2021 we assessed headstarted turtle health pre-release and 1 mo, 1 yr, and 2 yr after release using physical examination, hematology, plasma biochemistry, protein electrophoresis, and pathogen detection. Results were compared to wild-reared juveniles in the same habitats. Overall, 767 assessments from 561 turtles were included. Wild-reared and 2 yr post-release headstarts had higher incidence of hemoparasites, asymmetrical nares, and increased creatine kinase and aspartate aminotransferase activities (P<0.05) compared to all other groups. Erythrocyte sedimentation rate and heterophil:lymphocyte ratio were greater, while total leukocyte and lymphocyte counts were lower (P<0.05) in pre-release headstarts compared to wild-reared juveniles. Total solids, albumin, and beta globulins were higher, while the calcium:phosphorous ratio was lower (P<0.05) in pre-release headstarts and wild-reared juveniles vs. other groups. Bile acid levels were highest in pre-release headstarts (P<0.05). Body condition and gamma globulins increased following release, while alpha globulins and the albumin:globulin ratio decreased following release (P<0.05). Two pre-release and one post-release headstart tested positive for Emydomyces testavorans, one post-release headstart was positive for Mycoplasmopsis sp., and nine post-release turtles were positive for adenoviruses. Overall, rearing conditions have a profound and temporally dynamic impact on Blanding's health assessment parameters. Future studies should evaluate long-term impacts on morbidity and mortality to support positive health status and conservation outcomes.