Hematology of wild caught Dubois's tree frog Polypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae).

Pravati Kumari Mahapatra,Madhusmita Das

doi:10.1155/2014/491415

Abstract

Blood was analyzed from eighty (forty males and forty females) adult individuals of Polypedates teraiensis to establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

Highlights

Hematology is the most straightforward and less invasive technique to access the health status of natural population of vertebrates [1]
Detailed hematological reports on rhacophorid frog, Polypedates maculatus, have been studied by Mahapatra et al [8]. Another frog that lives sympatric with P. maculatus is P. teraiensis which is arboreal and mostly found in bushes, plantations, and gardens and rarely enters human habitation [9]
Blood cell profile of the tadpoles of P. teraiensis was described by us earlier [10] and here we describe the hematological and serum biochemical parameters of adults

Summary

Introduction

Hematology is the most straightforward and less invasive technique to access the health status of natural population of vertebrates [1] These parameters allow the detection of changes in physiological, pathological, ecological, and environmental conditions in natural population [2, 3]. Amphibians are known to be sensitive animals and show physiological variables to acute environmental changes [5] For this reason it has been suggested that physiological ecology of these animals should be incorporated into conservation plans and monitoring programs of individual populations [6]. Detailed hematological reports on rhacophorid frog, Polypedates maculatus, have been studied by Mahapatra et al [8] Another frog that lives sympatric with P. maculatus is P. teraiensis which is arboreal and mostly found in bushes, plantations, and gardens and rarely enters human habitation [9]. Blood cell profile of the tadpoles of P. teraiensis was described by us earlier [10] and here we describe the hematological and serum biochemical parameters of adults

Materials and Methods

Results and Discussion

Conclusion