Hematological indicators of mooses by means of automated analysis and microscopic method

Abstract

In recent years, automatic hematological analyzers have been introduced into veterinary practice for clinical blood analysis. In this regard, a number of reports have appeared in the literature reflecting the morphological parameters of elk blood obtained on automatic hemoanalyzers for veterinary and medical purposes. However, it should be noted that most of the veterinary equipment is programmed to conduct research on agricultural, small domestic, less often exotic animals, not the mooses. In addition, the semicytometric (impedancemetric) method of cell counting, which is the basis for the work of hemoanalyzers, unlike the classical (visual) method, allows to differentiate only three or five subpopulations of leukocytes. In this regard, the purpose of the research was to study the morphological parameters of blood using automatic analyzers in a comparative aspect with the visual method. In the first series of experiments on 17 moose calves of 1.5–2 months, a comparative assessment of hematological parameters was carried out by the microscopic method and on automatic analyzers (differentiating leukocytes into three subpopulations) for veterinary purposes RT-7600 (in the profiles "cow", "goat") and for medical purposes BC-3000 Plus. It has been found that the use of this equipment in the "cow" and "human" profiles makes it possible to evaluate the parameters of erythrocytes and platelets in moose. However, all indicators (except for hemoglobin) have significant discrepancies. Statistically significant discrepancies between the automatic determination and the visual method of leukocyte subpopulations were also found in four out of seven cases using the RT-7600 hemoanalyzer ("cow" profile) and in five out of seven cases using the BC-3000Plus hemoanalyzer. In the second series, seven adult animals were used to compare blood parameters obtained by the classical method and automatic – the Vet Scan HM5 hemoanalyzer in the profiles "cow", "sheep", "goat" (differentiating leukocytes into five subpopulations). It was revealed that there were no statistically significant differences in the characteristics of all parameters of erythrocytes in the profiles "cow", "sheep", except for the average content of hemoglobin in erythrocytes (pg). The "goat" profile did not allow us to evaluate the platelet parameters, while the erythrocyte parameters were close to the previous profiles. Determination of leukocytes and their subpopulations is possible only by a visual method and automated in the "cow" profile. In this regard, automatic hematology analyzers can be used to a certain extent in mass screening for red blood, and to a lesser extent – for white blood.