Abstract
Hematological characteristics of the anemia prevailing in wild Japanese flounder Paralichthys olivaceus were examined in detail by cell counting and microscopy. The erythrocyte counts, MCH, and MCHC decreased correlatively with the decrease in the hemoglobin concentration. Moreover, the reduction of erythrocyte volume was also observed in many anemic fish. Light microscopy showed erythrocytes with structural abnormalities, such as vacuolated, weakly stained cytoplasm and deformed outlines. In the TEM observations of erythrocytes, an insufficient accumulation of hemoglobin was observed as well. These results indicate that the anemia can be identified as a hypochromic microcytic anemia accompanied with hypoglobulia and a structural abnormality of the erythrocytes.
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