Hematological and Biochemical Investigations of Green Synthesized Reduced Graphene Oxide Carbon Nanoparticles on Acetaminophen Induced Male Albino Rats.

Abstract

The aim of this study is to evaluate the protective activity of green-synthesized Reduced graphene oxide carbon nanoparticles (RGO-CNP) from Asparagus racemosus (shatamuli) by hematological and biochemical assessments on acetaminophen induced uremic rats. 36 animals were divided into six groups including a control group, acetaminophen induced uremic group and nanoparticle treatment groups. Nanoparticle treated four groups were intraperitonealyco-administered RGO- CNP at doses of 0.5, 1.0, 2.0 and 4.0 mg/kg bw and acetaminophen at 500 mg/kg ofbw for 14 days respectively. Hematological analysis revealed that there was significant decrease (p<0.05) in Red Blood Cells (RBCs), Hemoglobin (Hb), with acetaminophen induced Group II. Co-administration of green synthesized RGO and acetaminophen on nanoparticle treating groups showed the effective protectivity and significant (p<0.05) recovery in the hematological alterations. Other hematological parameters including- WBC, Platelet, Hematocrit, MCH and MCHC remained within the reference range and no significant alterations were observed. There was a significant increase in plasma ALT, AST, serum urea and creatinine levels (p< 0.05) in uremic group (Group II) compare to control (Group I).The alterations were further resettled to the higher dose of RGO treated groups (Group V and VI). The other tested biochemical parameters ALP, total bilirubin, direct bilirubin, Serum triglyceride and cholesterol levels were not significantly altered when compared with control Group I. In conclusion, it is established that RGO possess protective response against acetaminophen induced toxicity as dose responsive manners, which is supported by haematological and biochemical observations. This promising effect suggests the potential applicability of RGO as therapeutics in drug delivery systems to reduce the toxicity.