Abstract
We have shown electronmicroscopically that HeLa Cell lines S3K and S3G undergo parallel changes in the morphology and composition of their cell surfaces when exposed to prednisolone. In response to this glucocorticoid derivative the clonal HeLa behave as if the property of contact inhibition is restored. A striking biochemical change characterizing this response is induction (S3G) or repression (S3K) of alkaline phosphatase activity. Interpretation of these changes rest in part on the amount and nature of the enzyme activity residing on the cell surface. Here we show that HeLa Cell surface alkaline phosphatase can be visualized in replica, and correlate this enzyme activity with specific surface features. A Gomori stain for alkaline phosphatase was combined with surface replication, using the approach described for visualizing lanthanum binding sites in replica.
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