Abstract
Radix Hedysari is an herbal preparation frequently used in traditional Chinese medicine. It can promote regeneration after peripheral nerve injury, but its effect on the amplification ratio (the ratio of distal to proximal fibers) during peripheral nerve regeneration has not yet been examined. In this study, we explored the effect of Hedysari extract on the amplification ratio in the peripheral nerve. Male Sprague-Dawley rats were separated into three groups at random: normal group (without surgery), model group (given sleeve nerve bridging surgery, but without adjuvant treatment) and treatment group (given sleeve nerve bridging surgery and then given Hedysari extract as adjuvant treatment). Twelve weeks after surgery, general observations, electrophysiological examination, histological analysis, morphometric measurements, and amplification ratio calculations were made. The results showed that nerve conduction velocity, the fiber and axon diameter, the g-ratio, the number of regenerating nerve fibers and the amplification ratio were better in the treatment group than in the model group, suggesting that Hedysari extract can effectively promote the growth of lateral buds in the proximal nerve stump and substantially improve the amplification effect during peripheral nerve regeneration.
Highlights
Traditional Chinese Medicine has been used in China for hundreds of years and plays an important role in the clinical setting
The exact active ingredients are not very clear (only some Hedysari polysaccharides (HPS) and mineral elements have been roughly detected from Hedysari extract [1]), it was found that Radix Hedysari has a major effect on the circulatory, urinary and immune systems
We sought to determine whether the use of traditional Chinese medicine as an adjuvant treatment after surgery could encourage proximal axons to grow more lateral buds and whether it could enhance the amplification effect
Summary
Traditional Chinese Medicine has been used in China for hundreds of years and plays an important role in the clinical setting. Radix Hedysari is the root of Hedysarum polybotrys Hand.-Mazz., and has tonifying, diuretic and circulatory effects It is blended into many Chinese medical formulations. Experimental studies have shown that Hedysari polysaccharides (HPS) can effectively promote peripheral nerve regeneration after nerve clamping injury, and can significantly improve the recovery of nerve function [2]. Proximal axons grow lateral buds toward the distal stump as a result of the growth promoting effects of various neurotrophic factors. The number of regenerated nerve fibers has not yet been sufficient for clinical functional recovery This requires us to identify a better method to promote the amplification effect. We sought to determine whether the use of traditional Chinese medicine as an adjuvant treatment after surgery could encourage proximal axons to grow more lateral buds and whether it could enhance the amplification effect. We investigated the effect of Hedysari extract on the amplification phenomenon, and found that Hedysari extract can effectively improve peripheral nerve regeneration by enhancing the amplification effect
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
