Abstract
Many studies have demonstrated a compensatory amplification phenomenon during nerve regeneration. When a relatively fine nerve is used as a donor to connect to a distal nerve after transection, the donor nerve regenerates more collaterals than its own fibers, which extend to the distal stump, grow into distal endoneurial tubes, and finally reach and dominate the target organs. This is known as the amplification phenomenon. In this study, we investigated the amplification phenomenon in rats treated with Modified Formula Radix Hedysari (MFRH) as adjuvant therapy for 12 weeks. The rats were divided into three groups at random (six animals in each group). In the model group and the treatment group, the proximal common peroneal nerve was used as a donor nerve to connect to the distal tibial nerve. Rats in the normal group did not undergo surgery. After surgery, the treatment group was administered MFRH as systemic therapy, while the model group and the normal group were not given treatment. The results demonstrated that the nerve conduction velocity, the fiber diameter, the axon diameter, the number of regenerating nerve fibers, and the amplification ratio were better in the treatment group than in the model group, suggesting that MFRH promoted the nerve amplification effect.
Highlights
Previous studies have shown that single axons can grow several new lateral buds in the initial stages of nerve regeneration
The total number of lateral buds that the proximal fibers grew was significantly more than the number of distal endoneurial tubes [1, 2]
Using fewer proximal fibers to bridge the distal nerve enabled the amplification phenomenon to be achieved during nerve regeneration, and the maximum amplification ratio for nerve regeneration was about 3.3
Summary
Previous studies have shown that single axons can grow several new lateral buds in the initial stages of nerve regeneration. Previous studies on nerve amplification phenomena have focused only on natural nerve growth and not on the effect of adjuvant therapy. To address this issue, we investigated whether Traditional Chinese Medicine as an adjuvant treatment after surgical operation can promote the amplification effect by inducing increased lateral bud growth on axons. Previous studies have shown that Hedysari Polysaccharides and Lumbricus extract could effectively promote peripheral nerve regeneration after nerve clamping injury and could significantly improve the recovery of nerve function [3, 4]. We further investigated the effect of MFRH on the amplification phenomenon
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