Heat stress impairs in vitro development of preantral follicles of cattle

Abstract

Although detrimental effects of heat stress on antral follicle development have been well studied, long-term effects - affecting the preantral follicle pool – are still largely unknown. The goal of this study was to evaluate effects of heat stress on growth, viability, gene expression and ATP production of preantral follicles of cattle. Follicles at the primary, early secondary and secondary stages were isolated from cattle ovaries and individually cultured while imposing physiological (CON; 38.5 °C) or intermittent heat stress (HS; 38.5 °C for 16 h and 41 °C for 8 h daily) conditions for 7 days. Individual follicles were subjected to real-time qPCR for determination of relative abundance of BAX, HSPA1A and SOD1 mRNA transcripts and evaluated for ATP production. Treatment for 7 days with intermittent HS decreased viability (P = 0.01) and diameter (P = 0.03) of preantral follicles. Relative abundances of BAX and HSPA1A mRNA transcripts were greater in follicles of the CON and HS groups that became non-viable during culture (P < 0.05); relative abundance of SOD1 mRNA transcript, however, was only greater in non-viable follicles of the HS group (P < 0.05), but not non-viable follicles of the CON group (P = 0.3). The ATP production was not different between viable follicles of the CON and HS group (P = 0.86). In conclusion, all stages of growing preantral follicles of cattle were susceptible to negative effects of heat stress. Follicles at the secondary stage of development were most sensitive, followed by early secondary and primary follicles.