Abstract
Poly(A) tail length (PAL) has been implicated in the regulation of mRNA translation activities. However, the extent of such regulation at the transcriptome level is less understood in plants. Herein, we report the development and optimization of a large-scale sequencing technique called the Assay for PAL-sequencing (APAL-seq). To explore the role of PAL on post-transcriptional modification and translation, we performed PAL profiling of Arabidopsis transcriptome in response to heat shock. Transcripts of 2,477 genes were found to have variable PAL upon heat treatments. Further study of the transcripts of 14 potential heat-responsive genes identified two distinct groups of genes. In one group, PAL was heat stress-independent, and in the other, PAL was heat stress-sensitive. Meanwhile, the protein expression of HSP70 and HSP17.6C was determined to test the impact of PAL on translational activity. In the absence of heat stress, neither gene demonstrated protein expression; however, under gradual or abrupt heat stress, both transcripts showed enhanced protein expression with elongated PAL. Interestingly, HSP17.6C protein levels were positively correlated with the severity of heat treatment and peaked when treated with abrupt heat. Our results suggest that plant genes have a high variability of PALs and that PAL contributes to swift posttranslational stress responses.
Highlights
As an essential environmental factor, temperature has far-reaching impacts on plant growth and development
We have developed and optimized a protocol termed APAL-seq (Assay for Poly(A) Tail Length - sequencing) that fulfills the gap between sequencing platforms and library construction by providing a transcriptome level poly(A) tail length measurement
APAL-seq can adjust the range of cDNA libraries by changing the amount of random primers present (Hodgson and Fisk, 1987)
Summary
As an essential environmental factor, temperature has far-reaching impacts on plant growth and development. This is especially true for land plants exposed to a wide range of temperature fluctuation daily and/or seasonally. Modern agriculture requires high crop productivity to meet increasing food demands. Even a small increase in temperature can lead to protein unfolding, mRNA Poly(A) Tail Length entanglement, and nonspecific aggregation. Mild heat stress has been shown to result in the reorganization of actin filaments into stress-defense forms. Severe heat stress results in the aggregation of vimentin or other filament-forming proteins, leading to the collapse of actin and tubulin networks (Welch and Suhan, 1985; Welch and Suhan, 1986)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
