Acute Heat Stress Induces the Differential Expression of Heat Shock Proteins in Different Sections of the Small Intestine of Chickens Based on Exposure Duration.

Sharif Hasan Siddiqui,Kwanseob Shim,Hyun Woo Choi,Jinryong Park,Darae Kang

doi:10.3390/ani10071234

Sharif Hasan Siddiqui, Kwanseob Shim + Show 3 more

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https://doi.org/10.3390/ani10071234

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Abstract

Simple SummaryThe small intestine is a pivotal organ in the alimentary tract of chickens. Approximately 90% of digestion and absorption occurs in the small intestine. The expression of heat shock proteins (HSPs) in different sections of the small intestine upon varying durations of heat exposure is unclear. In this study, we evaluated the protein and mRNA expression levels of HSP70, HSP60, and HSP47 in the duodenum, jejunum, and ileum, on acute heat exposure. The protein and gene expression of the HSPs in the different sections of the small intestine of chicken increased at different stages of acute heat stress and were reduced after the induction of heat tolerance. However, acute heat stress damaged the integrity of the different sections of the small intestine and liver in chickens.In this study, we examined the protein and gene expression of heat shock proteins (HSPs) in different sections of the small intestine of chickens. In total, 300 one-day-old Ross 308 broiler chicks were randomly allocated to the control and treatment groups. The treatment group was divided into four subgroups, according to the duration of acute heat exposure (3, 6, 12, and 24 h). The influence of heat stress on the protein and gene expression of HSP70, HSP60, and HSP47 in different sections of the small intestine of chickens was determined. The protein expression of HSP70 and HSP60 was significantly higher at 6 h in the duodenum and jejunum and 12 h in the ileum. The HSP47 protein expression was significantly higher at 3 h in the duodenum and ileum and at 6 h in the jejunum. The gene expression levels of HSP70, HSP60, and HSP47 were significantly higher at the 3 h treatment group than the control group in the duodenum, jejunum, and ileum. The glutamate pyruvate transaminase and glutamate oxaloacetate transaminase levels were significantly higher at 12 and 24 h in the serum of the blood. Acute heat stress affected the expression of intestinal proteins and genes in chickens, until the induction of heat tolerance.

Highlights

Climate change affects the poultry industry in tropical countries, and even in regions with a milder climate
Previous studies have indicated that the effects of heat stress on reduced organ function and the health of chickens can be confirmed by evaluating the changes in various serological parameters, such as serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) [4,5,6]
The expression levels of HSP70, HSP60, and HSP47 in the duodenum were significantly up-regulated at 6 h on acute heat stress, and were reduced after 6 h (Figure 1A–C)

Summary

Introduction

Climate change affects the poultry industry in tropical countries, and even in regions with a milder climate. Heat stress causes physiological and serological changes in chickens [3], which severely damage organs or tissues. Previous studies have indicated that the effects of heat stress on reduced organ function and the health of chickens can be confirmed by evaluating the changes in various serological parameters, such as serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) [4,5,6]. Acute heat stress induces the reduction of feed intake, and digestibility, and reduces the function of different organs in animals, resulting in the suppression of the growth rate [7,8,9]. The HSPs are highly conserved proteins that stimulate cytoprotection and induce heat tolerance in the cells of various organs and tissues, during the heat stress period [10,11]. One of the HSPs, HSP70, which is involved in the cellular response to different environmental stressors, might be a biomarker of the stress response [14]

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