Abstract
Okra (Abelmoschus esculentus L.) seeds and green tea (Camellia sinensis) have been known have many beneficial functions for human’s health, because of the high antioxidant contents and anti-cholesterol activities that can prevent cardiovascular disease. This research is aimed to make the formulation of healthy drink from okra seed juice with the addition of green tea extract. Green tea used was selected from green tea that has the best antioxidant activity from ten samples of green tea in the market. The best antioxidant activity data was found in Jawa tea extract with IC50 85.28±0.21 µg/mL which was formulated with the extract of okra seeds, made in the ratio of 1: 4. Selected healthy drinks were tested for physical characteristics, antioxidant activities, and sensory tests, and have antioxidant activity IC50 222.16±1.38 µg/mL. Further research was to analyze the potential of drinks in reducing cholesterol in vitro and in vivo ways. In vitro, 500 ppm drinks were able to inhibit cholesterol up to 47.55±0.76% and in vivo, there was a significant decrease in cholesterol levels after drink for 21 days with cholesterol reduction to 35.50±2.37%, triglycerides decrease 19.39±3.10%, LDL decrease 34.76±2.62%, and an increase of HDL to 94.74±16.53%.
Highlights
Okra plant was a kind of plant that has many benefits, and almost all parts of the plant such as leaves, flowers, stalks, fruit and seed which could be processed into food products
Statistical analysis that had been done indicating that different sample of green tea extract was giving significant (p
Green tea extract from Lipton Tea has the highest yield among the ten other green tea brands (7.98+0.15%)
Summary
Okra plant was a kind of plant that has many benefits, and almost all parts of the plant such as leaves, flowers, stalks, fruit and seed which could be processed into food products. Okra seeds have high protein content that can give hypocholesterolemic effects on various food products, and contain high levels of flavonols (±3.4 mg/g) as one of ingredients with a high phenolic component. Green tea was known for its high polyphenols content. The polyphenols contained in green tea include flavanol, hydroxyl-4flavanol, flavones, flavonoids, flavandiols and phenolic acids. These polyphenols contribute at least up to 30% of the dry weight of tea leaves and have a good contribution to human health which able to increase HDL levels in the blood that makes it good for preventing free radicals and preventing various kinds of diseases such as cholesterol. This research aimed to utilize Okra seeds and extract green tea that will be formulated into beverage that can reduce blood’s cholesterol (analysis in vitro and in vivo)
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