Healing effects of natural latex serum 1% from Hevea brasiliensis in an experimental skin abrasion wound model

Marcel Nani Leite,Saulo Nani Leite,Guilherme Ferreira Caetano,Thiago Antônio Moretti De Andrade,Márcio Fronza,Marco Andrey Cipriani Frade

doi:10.1016/j.abd.2019.12.003

Abstract

BackgroundDermabrasion is related with mechanical and surgical traumas on the skin; usually topical antiseptics and/or saline have been used for healing. Natural products for wound healing can also be used for abrasions, such as latex from Hevea brasiliensis. ObjectiveThis study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. MethodsFor in vitro evaluation, MTT and scratch assays were used. In vivo testing was performed in 72 rats submitted to dermabrasion, treated with saline, antiseptic, or latex serum. This study evaluated re-epithelialization, neutrophilic infiltration, and the quantification of crust and epidermis. ResultsLatex showed viability at 1% and 0.1% concentrations and migratory/proliferative activity at 0.01% concentrations. The re-epithelialization was highest in latex group on 7th day. The latex group displayed lower thickness of crusts and greater extent of epidermal layers. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day. Study limitationsAcute superficial wound model in rats and non-use of gel-cream (medium) without latex. ConclusionIn conclusion, non-toxic latex stimulated migration/proliferation of keratinocytes in vitro and significantly accelerated wound healing in animal excoriation models compared to chlorhexidine or saline.

Highlights

Cutaneous wounds are defined as the disruption of cellular and anatomical continuity of the skin and its functionality
The latex was obtained from a rubber tree (H. brasiliensis) of the clone RRhim 600
The in vitro viability of SLX was evaluated by the MTT colorimetric method using the NIH-3T3 fibroblast cell line and the human keratinocytes

Summary

Introduction

Cutaneous wounds are defined as the disruption of cellular and anatomical continuity of the skin and its functionality. Acute tissue losses can arise after dermatological procedures, such as dermabrasion and chemical peels, or can be caused by physical trauma, essentially reaching the epidermis and surface of the dermis. In this case, tissue repair occurs only through reepithelialization, followed by the process of anatomical and functional repair of the skin, resulting in an almost imperceptible scar. Objective: This study aimed to evaluate the in vitro viability and migratory/proliferative effects of latex serum from H. brasiliensis and to compare with a commercially available standard antiseptic solution and saline in experimental dermabrasion on rats. The latex and antiseptic groups showed increases of myeloperoxidase levels on the 2nd day and showed important reductions from the 7th day

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Discussion

Conclusion