Head and neck osteosarcoma: perineural invasion is associated with disease-free survival and tumor metastasis

Abstract

BackgroundHead and neck osteosarcoma (HNOS) is the most common bone malignancy in the head and neck region, accounting for 10% of all osteosarcoma cases. Perineural invasion (PNI) is a notable indication of aggressive tumor behavior, which includes the phenomenon of tumor cells invading any of the three layers of the nerve sheath or tumor cells gathering, encircling one-third of the nerve circumference, and infiltrating and metastasizing along the nerve. PNI has been reported in various malignant tumors and is considered to be linked to poor prognosis. PurposeThe study's purpose is to measure the association between PNI and survival outcomes in patients with HNOS. Study design, setting, sampleThis retrospective cohort study focused on HNOS patients who underwent surgery at the Department of Oral and Maxillofacial Head and Neck Oncology, Shanghai Ninth People’s Hospital School of Medicine, Shanghai Jiao Tong University, from 01/01/2019 to 31/12/2021. Patients who did not undergo complete surgical resection of the tumor, did not receive a conventional osteosarcoma diagnosis, and had positive surgical margins were eliminated. Predictor variableThe predictor variable is PNI status. The pathological section of the tumor was consistent with any of the PNI features, which was considered PNI-positive. Main outcome variable(s)The primary outcome variables were 3-year disease-free (DFS) and 3-year overall survival (OS). Secondary outcomes were 3-year tumor local recurrence (LR) and 3-year metastasis (MT). CovariatesCovariates were categorized into the following categories: demographic variables (age, sex), clinical variables (tumor region, primary tumor), and treatment variables (chemotherapy, radiotherapy). AnalysesAnalytic statistical methods were used for the data analysis. Pearson chi-square or fishier test was used to describe the baseline data. Kaplan-Meier is used to calculate survival rates. The Cox regression model was adapted for univariate and multivariate analysis. A p-value below 0.05 indicated statistical significance. ResultsThe study sample comprised 70 patients; 33 (47.1%) were male, and the mean age was 42.2 (SD:16.7) years old. There were 15 (21.4%) cases of PNI. The 3-year DSF rate and OS rate were 67.3% and 82.0%. PNI-positive resulted in higher risk for MT (P＜0.01, HR:5.95, 95% CI:1.62-21.86) and negative impact on DFS (P＜0.01, HR:6.35, 95% CI: 2.11-19.17) for HNOS patients. Conclusions and RelevancePositive PNI status was associated with decreased DFS and increased risk of MT.