HE EFFECT OF PROBIOTICS ON THE COLON MICROFLORA OF RATS IN CHRONIC HEART FAILURE

Abstract

Objective: to study the effect of long-term preliminary application of probiotics on the state of the rats` colon microflora in experimental chronic heart failure (CHF). Materials and methods. The experiments were performed on male rats (n = 38). CHF was modeled by double subcutaneous administration of isoprenaline hydrochloride (Sigma) at a dose of 80 mg/kg with 24-hour intervals. The composition of the intestinal microflora was determined on selective nutrient media with further incubation and subsequent evaluation of the results. Results. It has been found that the administration of drugs «Dialakt» and «Bifidumbacterin» decrease E.coli pool in normal conditions (p < 0.05) by 34.0 and 31.3 %, respectively. The CHF development leads to an increase of colibacillus titre by 46 % and decrease in the number of milk bacteria by 14.7 % and decrease of the antagonistic activity by 30 %. The prophylactic application of «Dialakt» and subsequent CHF modeling revealed that the number of E.coli was lower by 15.2% if to compare animals with CHF, the pool of Lactobacillus spp. was 28 % higher, and the pH value of the culture medium of bifidobacteria was lower than 4.5. The application of «Bifidumbacterin» leads to the same trend in the change of the analyzed parameters, which, however, does not reach the values of the background group. Conclusion. The preliminary use of «Dialakt» is conducive to maintain the balance of the intestinal microflora in chronic heart failure in rats and corresponds to the related indicators of the background group.