Abstract
Knowledge about cancer cell behavior on heterogeneous nanostructures is relevant for developing a distinct biomaterial that can actuate cancer cells. In this manuscript, we have demonstrated a harmonized approach of forming multi Ti-oxide phases in a nanostructure (MTOP nanostructure) for its unique cancer cell controlling behavior.Conventionally, single phases of TiO2 are used for targeted therapy and as drug carrier systems.In this research, we have shown a biomaterial that can control HeLa cells diligently using a combination of TiO, Ti3O and TiO2 phases when compared to fibroblast (NIH3T3) cells.MTOP-nanostructures are generated by varying the ionization energy in the vapor plume of the ultrashort pulse laser; this interaction with the material allows accurate tuning and composition of phases within the nanostructure. In addition, the lattice spacing of MTOP-nanostructures was analyzed as shown by HR-TEM investigations. An FESEM investigation of MTOP-nanostructures revealed a greater reduction of HeLa cells relative to fibroblast cells. Altered cell adhesion was followed by modulation of HeLa cell architecture with a significant reduction of actin stress fibers.The intricate combination of MTOP-nanostructures renders a biomaterial that can precisely alter HeLa cell but not fibroblast cell behavior, filling a void in the research for a biomaterial to modulate cancer cell behavior.
Highlights
Knowledge about cancer cell behavior on heterogeneous nanostructures is relevant for developing a distinct biomaterial that can actuate cancer cells
The irradiation from the ultrashort pulsed laser source was constituted by a 1040 nm wavelength direct-diode-pumped Yb-doped fiber amplified femtosecond laser system (Clark MXR) with an average power of 16W and repetition rate ranging from 4 MHz to 26 MHz.The titanium sample was mounted on a precision X-Y-Z stage normal to the ultrashort pulsed laser beam
When the energy delivered by the ultrashort pulsed laser is in excess of the binding energy of that atom, it breaks by means of repetitive laser pulses
Summary
Knowledge about cancer cell behavior on heterogeneous nanostructures is relevant for developing a distinct biomaterial that can actuate cancer cells. Regulating cancer cell behavior is a complex biological process, in which there is a need to restrain the cytoskeletal arrangement by bio-mimetic nano structured materials[1,2,3]. This communication is mediated by the direct interaction between cell surface receptors and physical extra cellular matrix (ECM) molecules. In order for cells to interact with nanotubes, their optimum diameter is 15nm, which indicates the limitations in releasing a drug for a longer duration[18,19]
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