Abstract

Conventionally, single phases of TiO2 are used for targeted therapy and a drug carrier systems. In this research a harmonized approach in synthesizing multi-Ti oxide phases in a nanostructure and its ability to control cancer cell cytoskeleton behavior. This modulation of HeLa cancer cell cytoskeleton behaviour including shape of the cell, surface area of the cell, alignment of the cell is diligent by using the combination of TiO, Ti3O, Ti2O phases. Field emission scanning electron microscope investigation (FESEM) revealed that multi-Ti oxide nanostructure revealed a greater reduction of HeLa cell relative to fibroblast cell. This altered cell adhesion was followed by modulation of HeLa cell architecture with significant reduction in actin stress fibers. The intricate combination of multi-Ti oxide nanostructures renders a biomaterial that can precisely alter HeLa cell but not the fibroblast cell behaviour has the potential application of creating a multi-Ti oxide nanostructure for targeted cancer therapy, developing nano patterning devices. This unique interaction of HeLa cancer cell with multi-Ti oxide nanostructure has provided an insight of cell-cell signalling which is the fundamental mechanism in regulating their proliferative characteristics.

Highlights

  • The dialogue between surface nanostructure materials and the cancer cell behaviour is a result of external biophysical stimulus, which is crucial for understanding many fundamental biological questions in designing biomaterial[44]

  • The results added an insight into understanding the phase dominance of titanium oxides in local cellular adhesion on multi-Ti oxide nanostructure even when their individual nanostructure morphology remains the same

  • Chapter – 4 HeLa cell patterning by ying-yang proliferative zones using multiphase titanium oxide nanomaterial synthesized by ultrashort pulsed laser

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Summary

Introduction

Regulating cancer cell behaviour is a complex biological process, where there is a need to restrain the cytoskeletal arrangement by bio-mimetic nano structured materials[12,39,40] This communication is mediated by the direct interaction between cell surface receptors and physical extra cellular matrix (ECM) molecules. Precise controlling of cellular microenvironments for the application of cancer cell engineering constructs, understanding the cellular interactions in diseased tissues and creating functional tissue replacements is the current problem in nanopatterning devices 71 These cellular communication in microenvironments aid us to understand the influence of spatial and geometric locations of cancer cell adhesion, proliferation, and metastasis 72. When HeLa cancer cells interact with nano bio-material formed at low peak power in longer pulse to pulse width they appeared to be not aligned, remained to have stable cell adhesion to the nanostructure. The surface chemistry of titanium nano biomaterial can provide deeper insight into the mechanism of cell-repelling when they come in contact with nano biomaterial and leads us to design an innovative non-drug induced altering cell behaviour

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