Abstract
Background: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing. Objective: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. Methods: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level. Results: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds. Conclusion: This system is definitely suitable for women’s point-of-care testing (POCT).
Highlights
Vagina is an essential micro-ecological region in women’s body [1]
Bacterial vaginitis is a very private disease and has a very high recurrence rate [4], Chinese women are often unwilling or have no time to go to the hospital for examinations due to traditional ideas, which may cause serious consequences [5]
Because the vaginal inflammation is mainly determined by the number of cocci, we only identified S. aureus at the same concentration (1 × 105 CFU/ml) in different proportions of the sample solution
Summary
Vagina is an essential micro-ecological region in women’s body [1]. Bacterial vaginitis is a very private disease and has a very high recurrence rate [4], Chinese women are often unwilling or have no time to go to the hospital for examinations due to traditional ideas, which may cause serious consequences [5]. It is crucial to develop a system that allows women to complete vaginal inflammation tests at home. Davis et al used Gram stain to detect bacterial vaginitis. They proposed that it is more sensitive to bacterial vaginosis than conventional smear microscopy [6]. Objective: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation. Conclusion: This system is definitely suitable for women’s point-of-care testing (POCT)
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