Abstract
Juvenile hormone esterase (JHE) activity and α-naphtyl acetate esterase (NacE) activity were studied in haemolymph of Diploptera punctata. The apparent K m value for JHE activity was 6.79 μM. Enzymatic activity of JHE and NAcE in females' haemolymph were measured during the first reproductive cycle. NAcE activity increased from emergence to oviposition and remained high during pregnancy. JHE activity was high at adult emergence and declined during the first three days. A sharp peak of JHE activity was observed on day 7 and high levels were maintained during pregnancy. JHE activity was not inhibited di isopropyfluorophosphate (DFP) whereas NAcEactivity was inhibited 90% at 1 mM. JHE activity profiles of virgin and allatectomized females did not show the peak on day 7 typical of normal mated females. Both oocyte growth and high JHE activity were restored in allatectomized females treated with hydroprene, a JH analogue, in a dose-dependent fashion. JH titre may regulate JHE activity at precise times during the reproductive cycle of D. punctata.
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