Abstract

Genetic lines of the cricket Gryllus assimilis selected for elevated vs. decreased activity of hemolymph juvenile hormone esterase (JHE) exhibited a 6.8-fold difference in enzyme activity by the seventh generation of selection. This documents that hemolymph JHE activity has the capability for rapid evolutionary change. This is the only insect endocrine trait for which such data are currently available. The difference in hemolymph JHE activity between the lines was due, to an equivalent degree, to variation in whole-cricket enzyme activity and allocation of JHE activity to the hemolymph compartment. No differences in kinetic or thermostability characteristics were observed between JHEs from the high- vs. low-selected lines. This suggests that the variation in JHE activity between the lines resulted from selection of variable regulatory genetic factors affecting the synthesis, degradation, or activation of enzyme activity. JHE and JH-binding activity in the hemolymph are genetically correlated, possibly due to co-regulation by the same genetic factors. Finally, in vivo JH degradation was significantly elevated in lines selected for elevated JHE activity. This documents that evolutionary changes in JHE activity alter in vivo JH metabolism. © 1996 Wiley-Liss, Inc.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.