Abstract
A modified procedure based on the cyanmethaemoglobin method for haemoglobin (Hb) determination in peanut nodules is described. It gave reliable results when tested on peanuts at all stages of vegetative growth, including when the green pigment was present in the nodules. It was shown that early (32–34 days after planting) determination of Hb in nodules of peanuts grown in a greenhouse under bacteriologically controlled conditions can be taken as a measure of the nitrogen-fixing ability of peanut Rhizobia and thus much of the time and greenhouse facilities usually required for strain screening may be conserved. Early determination of nitrogen concentration in leaflets of peanuts proved to be also a useful measure of Rhizobium efficiency, but less sensitive than the early determination of nodule haemoglobin.
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