Abstract

Photoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases. In this study, a new type of hydrogen peroxide (H2O[Formula: see text]-activated photoacoustic nanoprobe [Mn-AH nanoscale coordination polymer nanodots (NCPs)] was successfully synthesized by a simple one-step method in water phase containing 2,2[Formula: see text]-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), horse radish peroxidase (HRP), and manganese ion (Mn[Formula: see text]. After modification by polyethylene glycol (PEG), Mn-AH NCPs exhibited excellent stability and biocompatibility for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of endogenous H2O2. Using H2O2-activated Mn-AH NCPs, we have successfully performed PA imaging and H2O2detection of subcutaneous murine colon CT26 tumor and deep-seated orthotopic bladder tumor. Due to the inherent Mn element existence inside the Mn-AH, this nanoprobe also serves as a good T1-weighted magnetic resonance imaging (MRI) contrast agent simultaneously. Lastly, after accomplishing its imaging functions, the Mn-AH NCPs could be cleared out from the body without any long-term toxicity, providing a new opportunity for cancer diagnosis and treatment.

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